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Shanghai Genechem Ltd control shrna (shctrl) lentivirus
Control Shrna (Shctrl) Lentivirus, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna+(shctrl)+lentivirus/control+shrna++shnc++lentivirus/pmc10713595-215-5-17
Average 90 stars, based on 1 article reviews
control shrna (shctrl) lentivirus - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Hypoxia-autophagy axis induces VEGFA by peritoneal mesothelial cells to promote gastric cancer peritoneal metastasis through an integrin α5-fibronectin pathway
Article Snippet: For lentiviral production and infection, control shRNA (shCtrl/NC) lentivirus, shRNA against Atg7 (sh-Atg7), sgRNA against VEGFR1 (sgRNA-VEGFR1/FLT1), sgRNA against SIRT1 (sgRNA-SIRT1), sgRNA against Atg7 (sgRNA-Atg7) lentivirus and plasmids for Flag-p62, HA-SIRT1 N terminal domain (NTD, aa 1–234), sirtuin (catalytic) domain (SD, aa 234–510), C terminal domain (CTD, aa 510–747), full length (FL, aa 1–747) were purchased from Shanghai GeneChem Company.

Article Title: MDC1 functionally identified as an androgen receptor co-activator participates in suppression of prostate cancer
Article Snippet: For lentiviral production and infection, control shRNA (shCtrl) lentivirus and shRNA against MDC1 (shMDC1) lentivirus targeting the same sequence as siMDC1 as above were purchased from Shanghai GeneChem Company.

Article Title: Atg7 senses ATP levels and regulates AKT 1 -PDCD4 phosphorylation-ubiquitination axis to promote survival during metabolic stress
Article Snippet: For lentiviral production and infection, control shRNA (shCtrl) lentivirus, and shRNA against PDCD4 (shPDCD4) were purchased from Shanghai GeneChem Company.

Article Title: Calcium-binding and coiled-coil domain 2 promotes the proliferation and suppresses apoptosis of prostate cancer cells
Article Snippet: Human lentivirus-short hairpin (sh)CALCOCO2 and lentivirus-shControl (shCtrl) sequences were designed and purchased from Shanghai GeneChem Co., Ltd. Recombinant lentiviral vectors carrying CALCOCO2 shRNA were constructed using standard molecular techniques according to our previous study ( 21 ).

Article Title: The NAD-dependent deacetylase SIRT2 regulates T cell differentiation involved in tumor immune response
Article Snippet: In order to perform lentiviral production and infection, the control shRNA (shCtrl) lentivirus, shRNA against Sirt2 (shSirt2) and stably express Sirt2 lentivirus were purchased from Shanghai GeneChem Company.

Article Title: BAP18 coactivates androgen receptor action and promotes prostate cancer progression
Article Snippet: For lentiviral production and infection, control shRNA (shCtrl) lentivirus and shRNA against BAP18 (shBAP18) lentivirus targeting the same sequence as siBAP18#1 as above were purchased from Shanghai GeneChem Company.

Infection:

Article Title: ATM ‐ CHK 2‐Beclin 1 axis promotes autophagy to maintain ROS homeostasis under oxidative stress
Article Snippet: .. For lentiviral production and infection, control shRNA (shCtrl) lentivirus, shRNA against CHK2 (shCHK2), shRNA against Beclin 1 (shBeclin 1), and shRNA against FIP200 (shFIP200) lentivirus were purchased from Shanghai GeneChem Company. ..

Control:

Article Title: ATM ‐ CHK 2‐Beclin 1 axis promotes autophagy to maintain ROS homeostasis under oxidative stress
Article Snippet: .. For lentiviral production and infection, control shRNA (shCtrl) lentivirus, shRNA against CHK2 (shCHK2), shRNA against Beclin 1 (shBeclin 1), and shRNA against FIP200 (shFIP200) lentivirus were purchased from Shanghai GeneChem Company. ..

shRNA:

Article Title: ATM ‐ CHK 2‐Beclin 1 axis promotes autophagy to maintain ROS homeostasis under oxidative stress
Article Snippet: .. For lentiviral production and infection, control shRNA (shCtrl) lentivirus, shRNA against CHK2 (shCHK2), shRNA against Beclin 1 (shBeclin 1), and shRNA against FIP200 (shFIP200) lentivirus were purchased from Shanghai GeneChem Company. ..



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Shanghai Genechem Ltd control shrna (shctrl/nc) lentivirus
Hypoxia-induced autophagy mediated degradation of SIRT1 in PMCs promotes VEGFA secretion through acetylation of HIF-1α. a The levels of p62, LC3I/II, SIRT1, HIF-1α, and VEGFA were observed in HMrSV5 cells cultured in hypoxic conditions for the indicated time. b and c Western blot analysis of p62, LC3I/II, SIRT1, HIF-1α, and VEGFA under hypoxia and in response to treatment with chloroquine (CQ) or knockdown of ATG7 in HMrSV5 cells, or knockout of ATG7 in HEK293. d The levels of LC3I/II, p62, SIRT1, HIF-1α, and VEGFA were analyzed in HMrSV5 cells exposed to normoxia or hypoxia for 24 h with or without overexpression of SIRT1. e ATG7 was knocked out in HEK293 cells and knocked down in HMrSV5 cells, followed by exposure to normoxic or hypoxic conditions for 24 h . Immunoprecipitation was performed with a pan-acetyl antibody followed by immunoblot analysis using antibodies against HIF-1α. f Immunohistochemical analysis of LC3BI/II and VEGFA in benign mouse peritonea and GC metastatic peritonea. G. MGC-803 cells were subjected to normal media or conditioned media (CM1: conditioned media from hypoxic PMCs, CM2: conditioned media of hypoxic <t>shRNA-Atg7</t> PMCs) and to CM2 with synchronous addition of exogenous VEGFA. Representative photographs of adherent and migratory cells are shown. Scale bars represent 100 μm. Bars represent SD of the mean. * P < 0.05. ** P < 0.01. *** P < 0.001
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Hypoxia-induced autophagy mediated degradation of SIRT1 in PMCs promotes VEGFA secretion through acetylation of HIF-1α. a The levels of p62, LC3I/II, SIRT1, HIF-1α, and VEGFA were observed in HMrSV5 cells cultured in hypoxic conditions for the indicated time. b and c Western blot analysis of p62, LC3I/II, SIRT1, HIF-1α, and VEGFA under hypoxia and in response to treatment with chloroquine (CQ) or knockdown of ATG7 in HMrSV5 cells, or knockout of ATG7 in HEK293. d The levels of LC3I/II, p62, SIRT1, HIF-1α, and VEGFA were analyzed in HMrSV5 cells exposed to normoxia or hypoxia for 24 h with or without overexpression of SIRT1. e ATG7 was knocked out in HEK293 cells and knocked down in HMrSV5 cells, followed by exposure to normoxic or hypoxic conditions for 24 h . Immunoprecipitation was performed with a pan-acetyl antibody followed by immunoblot analysis using antibodies against HIF-1α. f Immunohistochemical analysis of LC3BI/II and VEGFA in benign mouse peritonea and GC metastatic peritonea. G. MGC-803 cells were subjected to normal media or conditioned media (CM1: conditioned media from hypoxic PMCs, CM2: conditioned media of hypoxic shRNA-Atg7 PMCs) and to CM2 with synchronous addition of exogenous VEGFA. Representative photographs of adherent and migratory cells are shown. Scale bars represent 100 μm. Bars represent SD of the mean. * P < 0.05. ** P < 0.01. *** P < 0.001

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Hypoxia-autophagy axis induces VEGFA by peritoneal mesothelial cells to promote gastric cancer peritoneal metastasis through an integrin α5-fibronectin pathway

doi: 10.1186/s13046-020-01703-x

Figure Lengend Snippet: Hypoxia-induced autophagy mediated degradation of SIRT1 in PMCs promotes VEGFA secretion through acetylation of HIF-1α. a The levels of p62, LC3I/II, SIRT1, HIF-1α, and VEGFA were observed in HMrSV5 cells cultured in hypoxic conditions for the indicated time. b and c Western blot analysis of p62, LC3I/II, SIRT1, HIF-1α, and VEGFA under hypoxia and in response to treatment with chloroquine (CQ) or knockdown of ATG7 in HMrSV5 cells, or knockout of ATG7 in HEK293. d The levels of LC3I/II, p62, SIRT1, HIF-1α, and VEGFA were analyzed in HMrSV5 cells exposed to normoxia or hypoxia for 24 h with or without overexpression of SIRT1. e ATG7 was knocked out in HEK293 cells and knocked down in HMrSV5 cells, followed by exposure to normoxic or hypoxic conditions for 24 h . Immunoprecipitation was performed with a pan-acetyl antibody followed by immunoblot analysis using antibodies against HIF-1α. f Immunohistochemical analysis of LC3BI/II and VEGFA in benign mouse peritonea and GC metastatic peritonea. G. MGC-803 cells were subjected to normal media or conditioned media (CM1: conditioned media from hypoxic PMCs, CM2: conditioned media of hypoxic shRNA-Atg7 PMCs) and to CM2 with synchronous addition of exogenous VEGFA. Representative photographs of adherent and migratory cells are shown. Scale bars represent 100 μm. Bars represent SD of the mean. * P < 0.05. ** P < 0.01. *** P < 0.001

Article Snippet: For lentiviral production and infection, control shRNA (shCtrl/NC) lentivirus, shRNA against Atg7 (sh-Atg7), sgRNA against VEGFR1 (sgRNA-VEGFR1/FLT1), sgRNA against SIRT1 (sgRNA-SIRT1), sgRNA against Atg7 (sgRNA-Atg7) lentivirus and plasmids for Flag-p62, HA-SIRT1 N terminal domain (NTD, aa 1–234), sirtuin (catalytic) domain (SD, aa 234–510), C terminal domain (CTD, aa 510–747), full length (FL, aa 1–747) were purchased from Shanghai GeneChem Company.

Techniques: Cell Culture, Western Blot, Knockdown, Knock-Out, Over Expression, Immunoprecipitation, Immunohistochemical staining, shRNA